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InviTrap® Spin Plant RNA Mini Kit
Opis
The sample is homogenized in liquid nitrogen and then lysed under highly denaturing conditions. The provided lysis buffers are adapted to the special requirements for lysis of plant material from different species. The genomic DNA is fixed at the surface of mineral carrier particles within the Lysis Solutions. Selective binding of the DNA is assured by optimized buffer conditions that reduce the undesired binding of RNA to a minimum. During the lysis procedure the carrier material is also shearing high molecular components, homogenizing the plant material and reducing the viscosity. After lysis the mineral carrier particles loaded with the genomic DNA are removed by centrifugation. Ethanol is added to provide appropriate binding conditions, then the sample is applied to a Spin Filter where the total RNA binds to the membrane and contaminants are efficiently washed away by repeated washing steps. The purified total RNA can be eluted in a small volume of RNase free water. Due to the high purity, the isolated total RNA is ready to use for downstream applications:
- RNA dot blots (Northern Blotting)
- cDNA transcription
- real-time PCR (quantitative RT-PCR, like TaqMan and LightCycler technologies)
- DDRT-PCR
- array technologies